| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Holdemania filiformis | CGAAGCAAGCGGTCGAAAC | AGGTCTTGCACAACGGCA | 60.15 | 59.81 | 288 | 76 |
100.00%
|
50.00%
|
| Primer 252 | Holdemania filiformis | GAAGCAAGCGGTCGAAACC | AGGTCTTGCACAACGGCA | 59.79 | 59.81 | 287 | 76 |
100.00%
|
50.00%
|
| Primer 253 | Holdemania filiformis | AAGCAAGCGGTCGAAACC | AGGTCTTGCACAACGGCA | 58.66 | 59.81 | 286 | 76 |
100.00%
|
50.00%
|
| Primer 254 | Holdemania filiformis | ATCGCAACGATCGGCTGT | ATGAAGAACGCCACCGCA | 59.82 | 59.97 | 251 | 76 |
100.00%
|
50.00%
|
| Primer 255 | Holdemania filiformis | CCGCAAAAGGCTTAACCGG | CCATCGCTGATTGTCTGGC | 59.79 | 58.98 | 159 | 76 |
100.00%
|
50.00%
|
| Primer 256 | Holdemania filiformis | TGTGCGACTTCAACGGCT | CGGTCGCTGTGCTGAACTA | 59.89 | 60.08 | 283 | 76 |
100.00%
|
50.00%
|
| Primer 257 | Holdemania filiformis | TGCTTCTGGTCATCGTCGG | TTAGTCGCCAAGCCTTCCG | 59.78 | 60.08 | 170 | 76 |
100.00%
|
50.00%
|
| Primer 258 | Holdemania filiformis | TGCGATCTTGGGCAGCAT | CCTGACCGGTGAAGTCGTT | 59.73 | 59.63 | 251 | 76 |
100.00%
|
50.00%
|
| Primer 259 | Holdemania filiformis | GCGGATTTTGCCCAGTGTC | ATGCACCGTGTTCAGGCA | 59.79 | 59.89 | 162 | 76 |
100.00%
|
50.00%
|
| Primer 260 | Holdemania filiformis | TCCGCACAACACGTTCCA | TCGTCCGTCGTTTCCGTT | 59.81 | 59.28 | 253 | 76 |
100.00%
|
50.00%
|